產(chǎn)品目錄

規(guī)格	1g 5g
CAS	62758-13-8
別名
化學(xué)名	sodium 7-oxido-3-oxo-3H-phenoxazine 10-oxide
分子式	C12H6NNaO4
分子量	251.17
溶解度	≥ 25.1mg/mL in DMSO
儲存條件	Store at -20°C
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition	Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request

產(chǎn)品描述：

The non-fluorescent resazurin has been used as a fluorogenic oxidation-reduction indicator in various cells by flow cytometry, fluorescence microscopy and high-throughput screening. Its red-fluorescent product, resorufin, has absorption/emission maxima ~575/585 nm.

In vitro: Resazurin has been applied as a simple and versatile way to measure cell proliferation and cytotoxicity. Although resazurin presented plenty of advantages over other cytotoxicity or proliferation tests, everal drawbacks to the routine use of resazurin were also observed. Previoius studies showed accumulation of the fluorescent product of resazurin in the medium which could result in an overestimation of cell population. Moreover, the extensive reduction of resazurin by metabolically active cells would lead to a final nonfluorescent product, and thus an underestimation of cellular activity [1]. In order to evaluate the effect of resazurin over longer incubation times, MCF7, MCF10A, 3T3-L1 and D1 cell lines were tested. Results showed that MCF7, 3T3-L1, and D1 cells cultured for 8 days with 20% resazurin had significantly less cell survivability. Moreover, levels of metabolic activity, DNA concentration, as well as glucose consumption of the cell lines in medium with 20% resazurin were significantly lower. Data also showed that the toxicities were more pronounced in the cancer cell lines than in the noncancerous cells [2].

特別提醒：

1. 本產(chǎn)品僅供科研使用。請勿用于醫(yī)藥、臨床診斷或治療，食品及化妝品等用途。請勿存放于普通住宅區(qū)。

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原創(chuàng)作者：上海莼試生物技術(shù)有限公司

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